Novel approaches to fight bacteria
From understanding the crossing of membrane barrier to the development of new nanotechnology-based drugs
July 10th – 14th, 2016
Jacobs University Bremen
Sunday, July 10th
| Arrival, transfer to the campus, all participants are hosted on campus, breakfast, lunch & dinner are organized in a reserved area of the College. Each morning/afternoon session is interrupted by a tea/coffee break in the Poster area, the posters will be displayed throughout the meeting. There will be sufficient time for poster discussions. | |
| 16:00 | Welcome desk (IRC foyer) |
| Introductions | |
| 17:00 | Malcolm Page (Jacobs University, Bremen, Germany)
What are the current bottlenecks in antibiotic research? |
| 17:45 | Robert Stavenger (GSK, Collegeville, USA)
Overview on the New drugs for bad bugs platform: Translocation & Enable |
| 18:00 | Iraida Loinaz (IK4-CIDETEC, Donostia/San Sebastián, Spain)
News from the EU project PneumoNP |
| 18:15 | Helena Bysell (SP Technical Research Institute of Sweden, Stockholm, Sweden)
News from the EU project FORMAMP |
| 18:30 | José Ainsa (Universidad de Zaragoza, Zaragoza, Spain)
News from the EU project NAREB |
| 18.45 | Mathias Winterhalter (Jacobs University, Bremen, Germany)
News from the ITN project Translocation |
| 20:00 | BBQ get-together & poster discussion
UEFA (the final match) screening on campus will be available for interested participants! |
Monday, July 11th
| 08:00 | Breakfast |
| Assays and mechanism of action – Chaired by Jean-Marie Pagès – | |
| 08:30 | Dirk Bumann (University of Basel, Basel, Germany)
What’s there: proteomic approach to understand the outer cell wall permeability |
| 09:00 | Juan Manuel Coya Raboso (Institut Pasteur, Paris, France)
Host and bacterial response to drug-loaded Nanoparticles |
| Nanoencapsulation: Strategies to improve performance of “new antibiotics” – Chaired by Helena Bysell – | |
| 09:30 | Marco Marradi (IK4-CIDETEC, Donostia/San Sebastián, Spain)
Polymeric nanoparticles for treating bacterial infections |
| 10:00 | Coffee break |
| 10:25 | Anita Umerska (Universite d’Angers, Angers, France)
Lipid nanoformulations as delivery vehicles for antimicrobial peptides |
| 10:50 | Dorothée Jary (CEA, Grenoble, France)
Lipid nanoparticles as delivery carriers for antibiotics |
| 11:15 | Mika Linden (Ulm University, Ulm, Germany)
Mesoporous silica nanoparticles as delivery vehicles for antimicrobial peptides |
| 11:40 | Laura de Matteis (Universidad de Zaragoza, Zaragoza, Spain)
Nanoemulsion-based chitosan nanocapsules as antibiotic delivery system |
| 12:05 | Randi Nordström (Uppsala University, Uppsala, Sweden)
Dendrimers and nanogels as delivery vehicles for antimicrobial peptides |
| 12:30 | Lunch |
| Development of novel assays to quantify transport across bacterial membranes
Mass-spectrometry & Fluorescence to quantify antibiotic uptake – Chaired by Dirk Bumann – |
|
| 14:30 | Jean-Marie Pagès (Aix-Marseille Université, Marseille, France)
Membrane barrier and drug translocation: Molecular approaches and imaging of antibiotic travel through bacterial envelope |
| 15:00 | Matthieu Réfrégiers (Synchrotron SOLEIL, Paris, France)
Looking at individual cell behaviour against antibiotics, one at a time |
| 15:30 |
Pamela Saint Auguste (University of Basel, Basel, Swizerland) Pseudomonas aeruginosa TonB-dependent transporters: in vivo expression and relevance |
| 16:00 | Coffee break |
| 16:30 |
Leopoldo Sitia (University of East Anglia, Norwich, UK) Nanoparticulate oligonucleotides for the treatment of drug-resistant pathogens: biodistribution and efficacy studies |
| 17:00 |
Silvia Acosta Gutierrez (University of Cagliari, Cagliari, Italy) Permeability in gram-negative bacteria: A microscopic journey |
| 17:30 | Mathias Winterhalter (Jacobs University, Bremen, Germany)
Permeation across porins |
| 18:00 | Miguel Vinas (University of Barcelona, Barcelona, Spain)
Antimicrobial peptides |
| 18:30 | Dinner |
| 19:30 | Heike Broetz-Oesterhelt (Eberhard Karls Universität Tübingen, Tübingen, Germany)
The bacterial cell envelope in antibiotic drug discovery |
| 20:00 | Poster session |
Tuesday, July 12th
| 08:00 | Breakfast |
| In vitro, ex vivo and in vivo animal models to evaluate the effect – Chaired by Malcolm Page & Barbara Cagniard – | |
| 08:30 | William J. Weiss (UNT Health Science Center, Fort Worth, USA)
The Use of Animal Models in Infectious Disease Research |
| 09:00 | Peter Warn (Evotec, Manchester, UK)
The rodent PKPD models to assist in dose selection for clinical trials |
| 09:30 | Abdessalem Rekiki (Bioaster, Paris, France)
MRSA infection: In vivo imaging and biodistribution of nanocarriers |
| 09:50 | Per Gerde (Inhalation Sciences Sweden AB, Stockholm, Sweden)
Ex vivo and in vivo models for evaluating effects of novel inhalation formulations |
| 10:10 | Coffee break |
| 10:40 | Jordi Llop (CIC BiomaGUNE, Donostia/San Sebastián, Spain)
Determination of regional distribution in the lungs using nuclear imaging |
| 11:00 | Hessel van der Weide (Erasmus Medical Centre, Rotterdam, The Netherlands)
Rat models of K. pneumoniae-ESBL and K. pneumoniae-KPC pneumonia, set-up and clinical validation |
| 11:20 | Sabine Wronski (Fraunhofer ITEM, Hannover, Germany)
Preclinical efficacy and safety testing of antimicrobial compounds |
| Translation to clinical development – Chaired by Marco Marradi – | |
| 11:40 | Magnus Strandh (Adenium Biotech, Copenhagen, Denmark)
Clinical development of antimicrobial peptides -opportunities and challenges from a SME perspective |
| 12:05 | Bruno Gonzales Zorn (Universidad Complutense de Madrid, Madrid, Spain)
Small-plasmid-mediated antibiotic resistance |
| 12:30 | Lunch |
| Understanding the impact of porin structure on antibiotic permeability – Chaired by Ulrich Kleinekathöfer & Matteo Ceccarelli – | |
| In this session we will discuss the role of porins in the permeability barrier imposed by the outer membrane of Gram-negative bacteria. How to correlate the recent high resolution structures to antibiotic transport, all atom modeling of molecular transport, novel assays for transport, recent cell-free assays to elucidate the permeation, current state of crystal structures and possible all atom modeling of the pathway. | |
| 14:30 | James H. Naismith (University of St. Andrews, St. Andrews, UK)
Progress in structural biology of outer membrane translocation |
| 15:00 | Matteo Ceccarelli (University of Cagliari, Cagliari, Italy)
Assessing permeability through bacterial porins |
| 15:30 | Bert Van den Berg (Newcastle University, Newcastle, UK)
Probing the portals: the OM diffusion channels of A. baumannii and P. aeruginosa |
| 16:00 | Coffee break |
| 16:15 | Ulrich Kleinekathöfer (Jacobs University, Bremen, Germany)
Molecular Simulations of Transport through Bacterial Nanopores |
| 16:45 | Wonpil Im (University of Kansas, Lawrence, USA)
New simulations in P. aeruginosa OM |
| 17:15 | Verena Fetz (Helmholtz Center for Infectious Research, Braunschweig, Germany)
MS-based quantification of small molecule uptake – setups and verification |
| 17:45 | |
| 18:30 | Dinner |
| 19:30 | Carolyn Shore (The Pew Charitable Trusts, Washington, USA)
A Roadmap for Antibiotic Discovery |
| 20:00 | Poster session |
Wednesday, July 13th
| 08:00 | Breakfast |
| Pro-drugging permeability (hijacking bacterial transport mechanisms) – Chaired by Malcolm Page & Paolo Ruggerone – | |
| A promising option to increase the uptake of antimicrobials is to subvert the function of specialized bacterial transport systems of essential nutrients (‘Trojan Horse’ strategy). Here our goal is to elucidate how siderophores or specific transporters of large hydrophilic molecules could be used for the uptake of antibiotics. | |
| 08:30 | Thilo Köhler (Université de Genève, Geneva, Switzeland)
Transport of siderophore-drug conjugates in Gram-negative bacteria |
| 08:55 | Gaëtan Mislin (University of Strasbourg, Strasbourg, France)
Enterobactin-dependent iron uptake pathway as a gate for antibiotic Trojan horse strategies against Pseudomonas aeruginosa |
| 09:20 | Jules Philippe (Jacobs University, Bremen, Germany) Sensing antibiotic permeability in Gram-negative bacteria |
| 09:45 | Emad Tajkhorshid (University of Illinois, Champaign, USA)
Microscopic Description and Structural Basis of the Mechanism of a Bacterial Drug Transporter |
| 10:15 | Coffee break |
| 10:30 | Nienke Buddelmeijer (Institut Pasteur, Paris, France)
The bacterial lipoprotein modification pathway – a promising drug target |
| 11:00 | Joe Eyermann (University of Cape Town, Cape Town, South Africa)
Permeability Challenges in the Hunt for New Tuberculosis Drugs |
| Efflux pumps – Chaired by Jürg Dreier & Helen I. Zgurskaya – | |
| 11:30 | Julien Buyck (University of Basel, Basel, Switzerland)
In vivo outer membrane proteome of pseudomonas aeruginosa: Role of simple porins |
| 12:00 | Helen I. Zgurskaya (University of Oklahoma, Norman, USA)
The broken barriers and their stories |
| 12:30 | Lunch |
| 13:30 | Klaas Martinus Pos (Goethe University Frankfurt, Frankfurt am Main, Germany)
Identification of drug binding determinants in the promiscuous efflux transport AcrB |
| 14:00 | Paolo Ruggerone (University of Cagliari, Cagliari, Italy)
Affinity sites in E. coli transporters: structural and dynamical features |
| 14:30 | Attilio Vittorio Vargiu (University of Cagliari, Cagliari, Italy)
Transport mechanism in the RND transporter AcrB of E. coli |
| 15:00 | Coffee break |
| 15:30 | Sandrasegaram Gnanakaran (Los Alamos National Laboratory, New Mexico, USA)
Targeting Efflux Pump Systems at Multiple Scales |
| ND4BB Data Hub: contribution to antibiotic research – Chaired by Robert Stavenger – | |
| This session presents rules/guidance for using the above detailed data in future drug discovery efforts (exploring the chemical space). | |
| 16:00 | Philip Gribbon (Fraunhofer IME ScreeningPort, Hamburg, Germany) |
| 18:30 | Dinner, round table, poster session |
Thursday, July 14th
| 08:00 | Breakfast |
| ITN Translocation Session – Chaired by Mathias Winterhalter – | |
| 09:00 | Pauline Maturana (Biozentrum Basel, Basel, Switzerland)
In vivo envelope stress responses of Salmonella |
| 09:20 | Luana Ferrara (University of St Andrews, St. Andrews, U.K.)
Structural studies of porins from Campylobacter jejuni and Enterobacter aerogenes |
| 09:40 | Vincent Trebosc (BioVersys AG, Basel, Switzerland)
A novel robust genome engineering platform to unravel pathogenic mechanisms in drug resistant Acinetobacter baumannii |
| 10:00 | Coffee break |
| 10:20 | Alessia Gilardi (Fraunhofer IME, Hamburg, Germany)
Identification of Small Molecules as Potential Adjuvants in Antibacterial Therapy |
| 10:40 | Jiajun Wang (Nanion Technologies GmbH, Munich, Germany)
The fluoroquinolone and Omp36 story |
| 11:00 | Monisha Pathania (Newcastle University, Newcastle upon Tyne, U.K.)
New porin structures |
| 11:20 | Venkata Krishnan (University of Cagliari, Monserrato, Italy)
Molecular rationale behind the differential substrate specificity of RND transporters AcrB and AcrD |
| 11:40 | Satya Prathyusha Bhamidimarri (Jacobs University, Bremen, Germany)
Understanding the permeation of substrate through a channel in Gram negative bacteria using electrophysiology |
| 12:00 | Koldo Morante (University of Tokyo, Tokyo, Japan)
Lipids as structural elements in pore formation |
| 12:30 | Lunch |
| 14:00 | IMI Translocation business meeting |
