Novel approaches to fight bacteria

From understanding the crossing of membrane barrier to the development of new nanotechnology-based drugs

July 10th – 14th, 2016
Jacobs University Bremen

Abstracts | Pictures

Sunday, July 10th

Arrival, transfer to the campus, all participants are hosted on campus, breakfast, lunch & dinner are organized in a reserved area of the College. Each morning/afternoon session is interrupted by a tea/coffee break in the Poster area, the posters will be displayed throughout the meeting. There will be sufficient time for poster discussions.
16:00 Welcome desk (IRC foyer)
Introductions
17:00 Malcolm Page (Jacobs University, Bremen, Germany)

What are the current bottlenecks in antibiotic research?

17:45 Robert Stavenger (GSK, Collegeville, USA)

Overview on the New drugs for bad bugs platform: Translocation & Enable

18:00 Iraida Loinaz (IK4-CIDETEC, Donostia/San Sebastián, Spain)

News from the EU project PneumoNP

18:15 Helena Bysell (SP Technical Research Institute of Sweden, Stockholm, Sweden)

News from the EU project FORMAMP

18:30 José Ainsa (Universidad de Zaragoza, Zaragoza, Spain)

News from the EU project NAREB

18.45 Mathias Winterhalter (Jacobs University, Bremen, Germany)

News from the ITN project Translocation

20:00 BBQ get-together & poster discussion

UEFA (the final match) screening on campus will be available for interested participants!

 

Monday, July 11th

08:00  Breakfast
Assays and mechanism of action – Chaired by Jean-Marie Pagès –
08:30 Dirk Bumann (University of Basel, Basel, Germany)

What’s there: proteomic approach to understand the outer cell wall permeability

09:00 Juan Manuel Coya Raboso (Institut Pasteur, Paris, France)

Host and bacterial response to drug-loaded Nanoparticles

Nanoencapsulation: Strategies to improve performance of “new antibiotics” – Chaired by Helena Bysell –
09:30 Marco Marradi (IK4-CIDETEC, Donostia/San Sebastián, Spain)

Polymeric nanoparticles for treating bacterial infections

10:00 Coffee break
10:25 Anita Umerska (Universite d’Angers, Angers, France)

Lipid nanoformulations as delivery vehicles for antimicrobial peptides

10:50 Dorothée Jary (CEA, Grenoble, France)

Lipid nanoparticles as delivery carriers for antibiotics

11:15 Mika Linden (Ulm University, Ulm, Germany)

Mesoporous silica nanoparticles as delivery vehicles for antimicrobial peptides

11:40 Laura de Matteis (Universidad de Zaragoza, Zaragoza, Spain)

Nanoemulsion-based chitosan nanocapsules as antibiotic delivery system

12:05 Randi Nordström (Uppsala University, Uppsala, Sweden)

Dendrimers and nanogels as delivery vehicles for antimicrobial peptides

12:30 Lunch 
Development of novel assays to quantify transport across bacterial membranes

Mass-spectrometry & Fluorescence to quantify antibiotic uptake 

– Chaired by Dirk Bumann –

14:30 Jean-Marie Pagès (Aix-Marseille Université, Marseille, France)

Membrane barrier and drug translocation: Molecular approaches and imaging of antibiotic travel through bacterial envelope

15:00 Matthieu Réfrégiers (Synchrotron SOLEIL, Paris, France)

Looking at individual cell behaviour against antibiotics, one at a time 

15:30

Pamela Saint Auguste (University of Basel, Basel, Swizerland)

Pseudomonas aeruginosa TonB-dependent transporters: in vivo expression and relevance

16:00 Coffee break
16:30

Leopoldo Sitia (University of East Anglia, Norwich, UK)

Nanoparticulate oligonucleotides for the treatment of drug-resistant pathogens: biodistribution and efficacy studies

17:00

Silvia Acosta Gutierrez (University of Cagliari, Cagliari, Italy)

Permeability in gram-negative bacteria: A microscopic journey

17:30 Mathias Winterhalter (Jacobs University, Bremen, Germany)

Permeation across porins

18:00 Miguel Vinas (University of Barcelona, Barcelona, Spain)

Antimicrobial peptides

18:30  Dinner
19:30 Heike Broetz-Oesterhelt (Eberhard Karls Universität Tübingen, Tübingen, Germany)

The bacterial cell envelope in antibiotic drug discovery

20:00 Poster session

 

Tuesday, July 12th

08:00  Breakfast
In vitro, ex vivo and in vivo animal models to evaluate the effect – Chaired by Malcolm Page & Barbara Cagniard –
08:30 William J. Weiss (UNT Health Science Center, Fort Worth, USA) 

The Use of Animal Models in Infectious Disease Research

09:00 Peter Warn (Evotec, Manchester, UK)

The rodent PKPD models to assist in dose selection for clinical trials

09:30 Abdessalem Rekiki (Bioaster, Paris, France)

MRSA infection: In vivo imaging and biodistribution of nanocarriers

09:50 Per Gerde (Inhalation Sciences Sweden AB, Stockholm, Sweden)

Ex vivo and in vivo models for evaluating effects of novel inhalation formulations

10:10 Coffee break
10:40 Jordi Llop (CIC BiomaGUNE, Donostia/San Sebastián, Spain)

Determination of regional distribution in the lungs using nuclear imaging

11:00 Hessel van der Weide (Erasmus Medical Centre, Rotterdam, The Netherlands)

Rat models of K. pneumoniae-ESBL and K. pneumoniae-KPC pneumonia, set-up and clinical validation

11:20 Sabine Wronski (Fraunhofer ITEM, Hannover, Germany)

Preclinical efficacy and safety testing of antimicrobial compounds

Translation to clinical development – Chaired by Marco Marradi –
11:40 Magnus Strandh (Adenium Biotech, Copenhagen, Denmark)

Clinical development of antimicrobial peptides -opportunities and challenges from a SME perspective

12:05 Bruno Gonzales Zorn (Universidad Complutense de Madrid, Madrid, Spain)

Small-plasmid-mediated antibiotic resistance

12:30 Lunch
Understanding the impact of porin structure on antibiotic permeability – Chaired by Ulrich Kleinekathöfer & Matteo Ceccarelli –
In this session we will discuss the role of porins in the permeability barrier imposed by the outer membrane of Gram-negative bacteria. How to correlate the recent high resolution structures to antibiotic transport, all atom modeling of molecular transport, novel assays for transport, recent cell-free assays to elucidate the permeation, current state of crystal structures and possible all atom modeling of the pathway.
14:30 James H. Naismith (University of St. Andrews, St. Andrews, UK)

Progress in structural biology of outer membrane translocation

15:00 Matteo Ceccarelli (University of Cagliari, Cagliari, Italy) 

Assessing permeability through bacterial porins

15:30 Bert Van den Berg (Newcastle University, Newcastle, UK) 

Probing the portals: the OM diffusion channels of A. baumannii and P. aeruginosa

16:00 Coffee break
16:15 Ulrich Kleinekathöfer (Jacobs University, Bremen, Germany)

Molecular Simulations of Transport through Bacterial Nanopores

16:45 Wonpil Im (University of Kansas, Lawrence, USA)

New simulations in P. aeruginosa OM

17:15 Verena Fetz (Helmholtz Center for Infectious Research, Braunschweig, Germany)

MS-based quantification of small molecule uptake – setups and verification

17:45
18:30 Dinner
19:30 Carolyn Shore (The Pew Charitable Trusts, Washington, USA)

A Roadmap for Antibiotic Discovery

20:00 Poster session

 

Wednesday, July 13th

08:00  Breakfast
Pro-drugging permeability (hijacking bacterial transport mechanisms) – Chaired by Malcolm Page & Paolo Ruggerone –
A promising option to increase the uptake of antimicrobials is to subvert the function of specialized bacterial transport systems of essential nutrients (‘Trojan Horse’ strategy). Here our goal is to elucidate how siderophores or specific transporters of large hydrophilic molecules could be used for the uptake of antibiotics.
08:30 Thilo Köhler (Université de Genève, Geneva, Switzeland) 

Transport of siderophore-drug conjugates in Gram-negative bacteria 

08:55 Gaëtan Mislin (University of Strasbourg, Strasbourg, France) 

Enterobactin-dependent iron uptake pathway as a gate for antibiotic Trojan horse strategies against Pseudomonas aeruginosa

09:20 Jules Philippe (Jacobs University, Bremen, Germany)
Sensing antibiotic permeability in Gram-negative bacteria
09:45 Emad Tajkhorshid (University of Illinois, Champaign, USA)

Microscopic Description and Structural Basis of the Mechanism of a Bacterial Drug Transporter

10:15 Coffee break
10:30 Nienke Buddelmeijer (Institut Pasteur, Paris, France) 

The bacterial lipoprotein modification pathway – a promising drug target

11:00 Joe Eyermann (University of Cape Town, Cape Town, South Africa) 

Permeability Challenges in the Hunt for New Tuberculosis Drugs

Efflux pumps – Chaired by Jürg Dreier & Helen I. Zgurskaya –
11:30 Julien Buyck (University of Basel, Basel, Switzerland)

In vivo outer membrane proteome of pseudomonas aeruginosa: Role of simple porins

12:00 Helen I. Zgurskaya (University of Oklahoma, Norman, USA) 

The broken barriers and their stories

12:30 Lunch
13:30 Klaas Martinus Pos (Goethe University Frankfurt, Frankfurt am Main, Germany) 

Identification of drug binding determinants in the promiscuous efflux transport AcrB

14:00 Paolo Ruggerone (University of Cagliari, Cagliari, Italy)

Affinity sites in E. coli transporters: structural and dynamical features

14:30 Attilio Vittorio Vargiu (University of Cagliari, Cagliari, Italy)

Transport mechanism in the RND transporter AcrB of E. coli

15:00 Coffee break
15:30 Sandrasegaram Gnanakaran (Los Alamos National Laboratory, New Mexico, USA)

Targeting Efflux Pump Systems at Multiple Scales

ND4BB Data Hub: contribution to antibiotic research – Chaired by Robert Stavenger –
This session presents rules/guidance for using the above detailed data in future drug discovery efforts (exploring the chemical space).
16:00 Philip Gribbon (Fraunhofer IME ScreeningPort, Hamburg, Germany)
18:30 Dinner, round table, poster session

 

Thursday, July 14th

08:00  Breakfast
ITN Translocation Session – Chaired by Mathias Winterhalter –
09:00 Pauline Maturana (Biozentrum Basel, Basel, Switzerland)

In vivo envelope stress responses of Salmonella

09:20 Luana Ferrara (University of St Andrews, St. Andrews, U.K.)

Structural studies of porins from Campylobacter jejuni and Enterobacter aerogenes

09:40 Vincent Trebosc (BioVersys AG, Basel, Switzerland)

A novel robust genome engineering platform to unravel pathogenic mechanisms in drug resistant Acinetobacter baumannii

10:00 Coffee break
10:20 Alessia Gilardi (Fraunhofer IME, Hamburg, Germany)

Identification of Small Molecules as Potential Adjuvants in Antibacterial Therapy

10:40 Jiajun Wang (Nanion Technologies GmbH, Munich, Germany)

The fluoroquinolone and Omp36 story

11:00 Monisha Pathania (Newcastle University, Newcastle upon Tyne, U.K.)

New porin structures

11:20 Venkata Krishnan (University of Cagliari, Monserrato, Italy)

Molecular rationale behind the differential substrate specificity of RND transporters AcrB and AcrD

11:40 Satya Prathyusha Bhamidimarri (Jacobs University, Bremen, Germany)

Understanding the permeation of substrate through a channel in Gram negative bacteria using electrophysiology

12:00 Koldo Morante (University of Tokyo, Tokyo, Japan)

Lipids as structural elements in pore formation

12:30 Lunch
14:00 IMI Translocation business meeting

 

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