Molecular basis of antibiotic permeability in Gram-negative bacteria

Solving a new question within a private-public partnership

June 21st – 23rd, 2017
Helmholtz Centre for Infection Research, Braunschweig, Germany

Abstracts | Pictures

Wednesday, June 21st

Arrival day
Introduction – Chaired by M. Winterhalter –
09:00 D. Heinz (Scientific Director of the Helmholtz Centre for Infection Research, Braunschweig, Germany)
Welcome and introduction to the Helmholtz Center for Infection Research
09:15 R. Stavenger (GlaxoSmithKline, Collegeville, PA, USA)
IMI TRANSLOCATION: Getting Drugs into Gram-Negative Bugs. Setting the Scene
09:45 H. Nikaido – keynote speaker (Professor of the Graduate School Division of Biochemistry, UC Berkeley, USA)
Can we predict drug permeation rates across the outer membrane?
10:30 Coffee break
11:00 M. Page (Jacobs University Bremen, Germany)
Illicit Drug Transport
Whole cell assays – Chaired by R. Stavenger & H. Schweizer –
11:30 J-M. Pagès & M. Masi (Aix-Marseille University, France)
Whole-cell-based Assay to Evaluate Permeation of ß-Lactam Antibiotics through OmpF/C porin orthologs
12:00 M. Réfrégiers (Synchrotron Soleil, Paris, France) & E. Dumont (Aix-Marseille University, France)
Microspectrofluorimetry dissection of the antibiotic accumulation in Gram-negative bacteria
12:30 M. Mourez (Sanofi-Aventis Lyon, France)
Why Translocation matters: Perspective from ongoing antibiotic discovery programs
13:00 Lunch & Poster session
15:30 Coffee break
16:00 M. Brönstrup (Helmholtz Zentrum Braunschweig, Germany)
Cellular assays based on mass spectrometry and microbiology to study drug uptake in Gram-negative bacteria
16:30 T. Köhler (Université de Geneve-Hopital, Switzerland)
The “ins and outs” of siderophore-mediated drug uptake in Gram-negative bacteria
17:00 M. A. Scorciapino (University of Cagliari, Italy)
Formation equilibria of complexes by siderophore-antibiotic conjugates with metal ions and their conformation investigated through NMR: the case of BAL30072
17:30 I. Schalk (Université Strasbourg)
Siderophore dependent iron uptake pathways in Pseudomonas aeruginosa: gates for antibiotic import
18:00 Dinner (on site)
19:00 L. Piddock (University of Birmingham, UK)
How understanding the biology of AcrAB-TolC helps drug discovery


Thursday, June 22nd

Morning talk – Chaired by M. Ceccarelli –
09:00 J. Naismith (University of St. Andrews, UK)
WP3 Structure based design of a Trojan horse
Porin Pathway – Chaired by M. Ceccarelli –
09:30 B. van den Berg (University of Newcastle, UK)
The importance of asymmetry
10:00 W. Im (Lehigh University, USA)
Modeling and Simulation of P. aeruginosa Outer Membrane and Proteins
10:30 Coffee break
Search for new molecules and strategies – Chaired by P. Gribbon & U. Kleinekathöfer –
11:00 A. Edwards (Structural Genomics Consortium, UK)
11:30 U. Kleinekathöfer (Jacobs University Bremen, Germany)
Transport across Bacterial Membranes: Insights from Molecular Simulations
12:00 M. Ceccarelli (University of Cagliari, Italy)
Bacterial Outer Membrane Porins as Electrostatic Nanosieves
12:30 S. Bhamidimarri & M. Winterhalter (Jacobs University Bremen, Germany)
Bacterial outer membrane permeability – Biophysical insight
13:00 Lunch & Poster session
15:30 Coffee break
16:00 D. Bumann (Biozentrum Basel, Swizerland)
In vivo modulation of Pseudomonas aeruginosa outer membrane permeability
16:30 B. Maier (Universität Köln, Germany)
A translocation ratchet drives DNA uptake during transformation
17:00 M. Zampieri (ETH Zürich, Switzerland)
The metabolic landscape of antibiotic modes of action
17:30 A. R. Brochado (European Molecular Biology Laboratory Heidelberg, Germany)
Species-specific activity of antibacterial drug combinations
18:00 Dinner (on site)
19:00 H. Brötz-Österhelt (Eberhard Karls Universität Tübingen, Germany)
Uptake of antibiotics across the cell envelope of E. coli


Friday, June 23rd

Morning talk – Chaired by J.-M. Pagès –
09:00 P. Gribbon (Fraunhofer IME Hamburg, Germany)
Data sharing in antibacterial research
Efflux systems – Chaired by L. Piddock –
10:00 C.-M. Lehr (Helmholtz Institute for Pharmaceutical Research Saarland, Germany)
Drug Delivery across Bacterial Barriers
10:30 Coffee break
11:00 B. Luisi (University of Cambridge, UK)
Structure of tripartite efflux pumps
11:30 H. Schweizer (University Florida, USA)
Efflux pumps in bacteria of the Burkholderia pseudomallei complex
12:00 J. Eyermann (University of Cape Town, South Africa)
Is it time to go back to the future?
12:30 M. Picard (CNRS Paris, France)
Investigation of a tripartite efflux pump from Pseudomonas aeruginosa: a bottom-up approach
13:00 Lunch
14:00 K. M. Pos (Universität Frankfurt, Germany)
Molecular basis of polyspecificity of drug efflux pumps
14:30 P. Ruggerone (University of Cagliari, Italy)
Structural and dynamical determinats of molecule-RND transporter interactions
15:00 M. Winterhalter (Jacobs University Bremen, Germany)
Concluding remarks